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The intensity data were subsequently translated into molar concentrations by referencing a pre-calibrated standard curve

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Following translation of the mRNA into proteins, and no cellular survival initiative of growth factors, FOXOs translocate to the nucleus upregulating a series of target genes of the cell cycle, stress resistance, and longevity

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RT-qPCR was performed with the LightCycler 480 system (Roche), following the manufacturer's instruction with SYBR Premix Ex Taq (TaKaRa)