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Glutathione disulfide production during arachidonic acid oxygenation in human platelets

RT was done with a RevertAid H cDNA Synthesis Kit (Thermo Fisher Scientific, USA) according to manufacturers instructions using both TMV coat protein gene reverse primer ( TMV-CP rev) and an oligo-dT primer

After the measurement, cells were lysed with 0.1% Triton X-100 and stained with SYBR Green (S9430, Sigma-Aldrich) in the well

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