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Recommended Aseptic Practices Single-use needles : Never reuse needlesrepeated punctures damage rubber stoppers, allowing microbial ingress and losing vacuum seal Alcohol swabbing : Swab stopper before each puncture and allow alcohol to fully evaporate (30 seconds) before inserting needle Syringe selection : Use luer-lock syringes rather than slip-tip to prevent accidental needle detachment during withdrawal Withdrawal technique : Insert needle at a slight angle to minimize coring of rubber stopper particles into solution Air displacement : Inject an equal volume of air into the vial before withdrawing liquid to maintain neutral pressurethis prevents stopper collapse in multi-dose vials Signs of Microbial Contamination Even with bacteriostatic water, gross contamination can occur with poor technique: Cloudiness that develops over 24-48 hours (peptide precipitation is immediate

IMG_020440323024 3.11 MB ncsugrad2002 June 30, 2019, 2:45pm 2 You can always add a ml or 2 and see how full it is

& Swanson, R

J Membrane Biol 256 , 223228 (2023)

Together we can all come out on the other side of this experience healthier, happier and more centered with better habits we can share in together

Christine serves patients at Bellevue Square and Nordstrom Bellevue