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Quantitative real-time polymerase chain reaction (qPCR) was performed with TaqMan Gene Expression Assays, according to the manufacturers protocol using TaqMan primers for UCP1 (Hs00222453_m1), UCP2 (Hs01075227_m1), NAMPT (Hs00237184_m1), and FNDC5 (Hs00401006_m1) (Applied Biosystems, Carlsbad, CA, USA)