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Diabetes Care 31 , 21882192 (2008)

The SGBS cells were cultured in a basal medium of DMEM/F12 medium supplemented with 10% FBS, 3.3 mM biotin,1.7 mM pantothenic acid, and 100 U/ml penicillin-streptomycin (Sigma-Aldrich)

An explanation of how we came to these new recommendations can be found in Vitamin B12: Rationale for Recommendations

al, The Effect of Medium-Term Sauna-Based Heat Acclimation (MPHA) on Thermophysiological and Plasma Volume Responses to Exercise Performed under Temperate Conditions in Elite Cross-Country Skiers