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Notably, no significant changes in bone turnover markers (osteocalcin, bone-specific alkaline phosphatase) were observed, indicating effects primarily through bone volume rather than remodeling markers

For calcium imaging studies, male Naval Medical Research Institute (NMRI) mice (Taconic Biosciences, BomTac:NMRI) were used (aged 1828 days)

Both L-ascorbic acid (Vitamin C) and retinoids thrive in a low-pH (acidic) environment, while GHK-Cu is most stable and effective at a more neutral pH