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This study aimed to evaluate the efficacy of HPE to reduce MTX-induced nephrotoxicity

When the research question specifically isolates these two mechanisms, adding GHK-Cu introduces confounding from its gene-expression and anti-inflammatory effects

Standard lab handling: Use typical peptide handling precautions and follow institutional biosafety procedures

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Each visit combines three targeted peptides: CJC-1295 DAC, KPV, MOTS-c

GSH levels were calculated both relative to Cr (GSH/Cr peak area ratio) and as absolute (water-referenced) GSH concentrations