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The cells were washed three times with secretion buffer (4.5 mM KCl, 138 mM NaCl, 4.2 mM NaHCO 3 , 1.2 mM NaH 2 PO 4 , 2.6 mM CaCl 2 , 1.2 mM MgCl 2 , and 10 mM HEPES, which was adjusted to pH 7.4 with NaOH) supplemented with 0.1% fatty acid-free bovine albumin serum (BSA

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Results showed that metformin could increase p-AMPK protein level in a dose-dependent manner, whereas liraglutide did not have such effect as metformin (Supplemental Fig

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Based on this finding, all Phase 3 TRIUMPH trials use 2 mg as the universal starting dose, with the first escalation to 4 mg occurring at week 5

Recheck after 15 minutes