Estrogen, a female hormone, can have a protective effect on the hair follicles
Freezing can cause the peptide to precipitate out of solution, forming particles that do not re-dissolve upon thawing
You should strive for a minimum of seven hours of sleep each night
Immunostaining After organotypic culture or cryosection, the cochlear explants or tissue sections were fixed with 4% PFA, permeabilized with 1% TritonX-100 in PBS, and blocked by incubation in PBT-1 solution (0.1% Triton X-100, 8% donkey serum, 1% bovine serum albumin, and 0.02% sodium azide in PBS) at room temperature for 1 h
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