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On the day of the assay, serial dilutions (7-fold dilutions, 7 concentrations per compound and one well containing only assay buffer) of test compounds were performed in duplicate in assay buffer to appropriate concentrations in 96-well plates (Greiner, U-shape #GR-650201) using a Biomek i7 liquid handler

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Structurally, BPC-157 is small enough to be synthesized at scale, stable enough at gastric pH to survive oral administration in some experimental conditions, and short enough that the immune system rarely treats it as foreign

PMID: 26500843

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