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Vigorous agitation creates foam and can denature the peptide through mechanical shearing

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(EtherPC-1 n = 14 metabolites, EtherPC-2 n = 90 metabolites, EtherPE-1 n = 58 metabolites, EtherPE-2 n = 67 metabolites, FA-1 n = 15 metabolites, FA-2 n = 29 metabolites, PC-1 n = 62 metabolites, PC-2 n = 51 metabolites, PG-1 n = 55 metabolites, PG-2 n = 45 metabolites, TG-1 n = 10 metabolites, TG-2 n = 96 metabolites)

[2] Timing is crucial for optimal effectiveness

Lusini, L